Important Publications at Science

Microfluidic Digital PCR Enables Multigene Analysis of Individual Environmental Bacteria
Elizabeth A. Ottesen, Jong Wook Hong, Stephen R. Quake, and Jared R. LeadbetterScience 1 December 2006 314: 1464-1467 [DOI: 10.1126/science.1131370] (in Reports)
......Reports MICROBIO Microfluidic Digital PCR Enables Multigene Analysis of Individual...microfluidic digital polymerase chain reaction (PCR) to amplify and analyze multiple, different...catalyzing important activities in situ ( 1 ). PCR-based techniques that use single genes as......
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DATABASE: Get Primed for PCR
Science 30 July 2004 305: 585 [DOI: 10.1126/science.305.5684.585b] (in NetWatch)
...netwatch NET LINK NW_MOLEC BIOL DATABASE: Get Primed for PCR Researchers use reverse transcription PCR to measure amounts of mRNA in a cell and gauge gene activity. Find the right primers for a particular......
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PCR in a Rayleigh-Bénard Convection Cell
Madhavi Krishnan, Victor M. Ugaz, and Mark A. BurnsScience 25 October 2002 298: 793 [DOI: 10.1126/science.298.5594.793] (in Brevia)
...Brevia BIOCHEM PCR in a Rayleigh-B e a nard Convection...convection to perform polymerase chain reaction (PCR) amplification of DNA inside a 35-Ml cylindrical...the diameter of the cavity. In the case of PCR, the required reaction efficiency constrains......
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PCR:Roche Dealt a Setback on European Taq Patent
Robert F. ServiceScience 8 June 2001 292: 1815 [DOI: 10.1126/science.292.5523.1815a] (in News of the Week)
...n-week EUROPE NEWS SCI BUSINESS PCR: Roche Dealt a Setback on European Taq Patent Robert F. Service PCR. Roche dealt a setback on European Taq patent...Recombinant Proteins Taq Polymerase PCR:Roche Dealt a Setback on European Taq Patent......
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PCR:Taq Polymerase Patent Ruled Invalid
Robert F. ServiceScience 17 December 1999 286: 2251-2253 [DOI: 10.1126/science.286.5448.2251b] (in News of the Week)
...PCR: Taq Polymerase Patent Ruled Invalid Robert F. Service...polymerase, is a crucial element of the polymerase chain reaction (PCR), the ubiquitous technique used to replicate snippets of DNA...Taq market, but Promega is challenging Roche's patents on PCR itself and may do the same with other Taq patents Roche holds......
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INFECTIOUS DISEASE:PCR Detection of Bacteria in Seven Minutes
Phillip Belgrader, William Benett, Dean Hadley, James Richards, Paul Stratton, Raymond Mariella Jr., and Fred MilanovichScience 16 April 1999 284: 449-450 [DOI: 10.1126/science.284.5413.449] (in Tech.Sight)
...INFECTIOUS DISEASE: PCR Detection of Bacteria in Seven Minutes...bacterial tests use polymerase chain reaction (PCR) because of its demonstrated effectiveness...For field use, a variation of standard PCR called real-time PCR is the most practical......
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SIGHTINGS:PCR on a Roller Coaster
Richard Peters and Robert SikorskiScience 19 June 1998 280: 1956 [DOI: 10.1126/science.280.5371.1956a] (in Tech.Sight)
...SIGHTINGS: PCR on a Roller Coaster Techwire Using...this topic. The polymerase chain reaction (PCR) has been a wildy successful and commonly...delays allowed the researchers to perform 20 PCR cycles in about 4 min and yield sample quality......
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Performing PCR on a chip
Science 15 May 1998 280: 977 [DOI: 10.1126/science.280.5366.977c] (in This Week in Science)
...The three steps of the polymerase chain reaction (PCR), melting the double-stranded DNA, binding specific primers, and enzymatically extending the primers, have been automated on a......
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Chemical Amplification: Continuous-Flow PCR on a Chip
Martin U. Kopp, Andrew J. de Mello, and Andreas ManzScience 15 May 1998 280: 1046-1048 [DOI: 10.1126/science.280.5366.1046] (in Reports)
...Chemical Amplification: Continuous-Flow PCR on a Chip Martin U. Kopp...to perform the polymerase chain reaction (PCR) in continuous flow at high speed. The device...independent of input concentration. A 20-cycle PCR amplification of a 176-base pair fragment......
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SCIENCE AND COMMERCE:PCR Patent Tangle Slows Quick Assay of HIV Levels
Jon CohenScience 6 June 1997 276: 1488-1491 [DOI: 10.1126/science.276.5318.1488a] (in News & Comment)
...SCIENCE AND COMMERCE: PCR Patent Tangle Slows Quick Assay of HIV Levels...rights to the polymerase chain reaction (PCR), the basic analytic technique on which...Systems Inc., which holds the patent on PCR, PE Applied Biosystems cannot promote diagnostic......
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Book ReviewsMaking PCR and Exquisite Specificity, reviewed by S. Hilgartner * Chondrules and the Protoplanetary Disk, H. Y. McSween Jr. * Biotic Recovery from Mass Extinction Events, J. H. Lipps * Vignette * Books Received
Science 8 November 1996 274: 934-936 [DOI: 10.1126/science.274.5289.934] (in Departments)
...Making PCR. A Story of Biotechnology. PAUL RABINOW. University...antibodies and the polymerase chain reaction (PCR) are widely regarded as among the most important...enjoy a vast market, and the patent rights to PCR sold in 1989 for a reported $300 million......
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Scientists named in PCR suit
M BarinagaScience 2 June 1995 268: 1273 [DOI: 10.1126/science.7761847] (in Articles)
......Race-Twice Rachel Nowak Scientists named in PCR suit. News EC 2.7.7.- Taq Polymerase...Rachel Nowak BIOTECH PATENTS Scientists Named in PCR Suit Ifyour lab uses the polymerase chain reac-tion (PCR), yourname may have come up in a San Francisco......
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PCR products and CITES
M JonesScience 23 December 1994 266: 1930 [DOI: 10.1126/science.7801116] (in Articles)
...articles PCR products and CITES M Jones PCR products and CITES. Comment Letter 9007-49-2 DNA Science...org (for submitting classi-fied advertisements) 1930 LETTERS PCR Products and CITES Brian Bowen and John Avise's letter, "Con......
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Conservation research and the legal status of PCR products
BW Bowen and JC AviseScience 4 November 1994 266: 713 [DOI: 10.1126/science.7973619] (in Articles)
......Conservation research and the legal status of PCR products BW Bowen JC Avise Conservation research and the legal status of PCR products. Letter 9007-49-2 DNA Science...Conservation Research and the Legal Status of PCR Products The challenge of obtaining biological......
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Advanced PCR
Mary Ann D. BrowScience 5 August 1994 265: 817-819 [DOI: 10.1126/science.265.5173.817-a] (in Articles)
......of Science Article Article Advanced PCR Mary Ann D. Brow Third Wave Technologies...Madison, WI 53711-5368, USA Advanced PCR. JOURNAL ARTICLE Modern Cosmology and...Washington, Seattle, WA 98195, USA a Advanced PCR The Polymerase Chain Reaction. KARY B. MULLIS......
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'Long PCR' leaps into larger DNA sequences
J CohenScience 18 March 1994 263: 1564-1565 [DOI: 10.1126/science.8128242] (in Articles)
...articles 'Long PCR' leaps into larger DNA sequences J Cohen 'Long PCR' leaps into larger DNA sequences. News 0 DNA Primers...business," he says.-Faye Flam MOLECULAR BIOLOGY 'Long PCR' Leaps Into Larger DNA Sequences On Christmas Eve 1992......
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Hantavirus outbreak yields to PCR
E MarshallScience 5 November 1993 262: 832-836 [DOI: 10.1126/science.8235602] (in Articles)
...articles Hantavirus outbreak yields to PCR E Marshall Hantavirus outbreak yields to PCR. News Science. 1993 Nov 5;262(5135):850-1...ICVVP~~~ a UIIIFEII Hantavirus Outbreak Yields to PCR Researchers have yet to isolate the virus that......
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PCR enzyme. The more you use, the cheaper it gets
P AldhousScience 6 August 1993 261: 678 [DOI: 10.1126/science.8342033] (in Articles)
...articles PCR enzyme. The more you use, the cheaper it gets P Aldhous PCR enzyme. The more you use, the cheaper it gets. News...economics Switzerland Taq Polymerase United States PCR ENZYME The More You Use, the Cheaper It Gets Big users......
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Detection of HIV-1 DNA and messenger RNA in individual cells by PCR-driven in situ hybridization and flow cytometry
BK Patterson, M Till, P Otto, C Goolsby, MR Furtado, LJ McBride, and SM WolinskyScience 14 May 1993 260: 976-979 [DOI: 10.1126/science.8493534] (in Articles)
......and messenger RNA in individual cells by PCR-driven in situ hybridization and flow cytometry...and messenger RNA in individual cells by PCR-driven in situ hybridization and flow cytometry...and Messenger RNA in Individual Cells by PCR-Driven in Situ Hybridization and Flow Cytometry......
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Immuno-PCR with a commercially available avidin system
V Ruzicka, W Marz, A Russ, and W GrossScience 30 April 1993 260: 698-699 [DOI: 10.1126/science.8480182] (in Articles)
...articles Immuno-PCR with a commercially available avidin system...Ruzicka W Marz A Russ W Gross Immuno-PCR with a commercially available avidin system...GTCCCCAGTCATCAGCTCCTG-3' and 5'-AGAGTTGCATGCCATGGTCCC-3'). PCR reac-tions (1 00-pI) were done with biotinylated......
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PCR enzyme patent challenged
P AldhousScience 23 April 1993 260: 486 [DOI: 10.1126/science.8475381] (in Articles)
......s Very Own Red Scare Wayne Biddle PCR enzyme patent challenged. News EC 2...biography ofvon Braun. INTELLECTUAL PROPERTY PCR Enzyme Patent Challenged Thesparkshave begun...make use of the polymerase chain reaction (PCR)-the gene-amplifying technique that's one......
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PCR Enzyme Patent Challenged
Peter AldhousScience 23 April 1993 260: 486 [DOI: 10.1126/science.260.5107.486-a] (in Articles)
......Advancement of Science Article Article PCR Enzyme Patent Challenged Peter Aldhous...biography ofvon Braun. INTELLECTUAL PROPERTY PCR Enzyme Patent Challenged Thesparkshave begun...make use of the polymerase chain reaction (PCR)-the gene-amplifying technique that's one......
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High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR
M Piatak, Jr, MS Saag, LC Yang, SJ Clark, JC Kappes, KC Luk, BH Hahn, GM Shaw, and JD LifsonScience 19 March 1993 259: 1749-1754 [DOI: 10.1126/science.8096089] (in Articles)
......stages of infection determined by competitive PCR M Piatak Jr MS Saag LC Yang SJ Clark...competitive polymerase chain reaction (QC-PCR) methods were used to quantify virion-associated...therapy. Plasma virus levels determined by QC-PCR correlated with, but exceeded by an average......
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Roche gets tough on illicit sales of PCR reagent
P AldhousScience 4 December 1992 258: 1572-1573 [DOI: 10.1126/science.1455243] (in Articles)
...articles Roche gets tough on illicit sales of PCR reagent P Aldhous Roche gets tough on illicit sales of PCR reagent. News 0 Indicators and Reagents...REACrION Roche Gets Tough on Illicit Sales of PCR Reagent PCRusers beware. Ifyou have been......
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Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates
T Sano, CL Smith, and CR CantorScience 2 October 1992 258: 120-122 [DOI: 10.1126/science.1439758] (in Articles)
...articles Immuno-PCR: very sensitive antigen detection by means...immuno-polymerase chain reaction (immuno-PCR), was developed in which a specific DNA...segment of the attached DNA was amplified by PCR. Analysis of the PCR products by agarose......
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PCR Regulations
Science 21 February 1992 255: 927 [DOI: 10.1126/science.255.5047.927-c] (in Articles)
......Advancement of Science ARTICLE ARTICLE PCR Regulations Parting blast from AIDS official...teachersinyourchildren's science classrooms. PCR Regulations Following up on its promise...the use ofthe polymerase chain reac-tion (PCR)(Science,31 January, p. 528), Hoffmann-La......
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PCR amplification of specific alleles
SS SommerScience 31 January 1992 255: 514 [DOI: 10.1126/science.1736349] (in Articles)
...articles PCR amplification of specific alleles SS Sommer PCR amplification of specific alleles. Comment Letter...genetics Polymerase Chain Reaction Restriction Mapping PCR Amplification of Specific Alleles Rick Weiss' Research......
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Roche eases PCR restrictions
M HoffmanScience 31 January 1992 255: 528 [DOI: 10.1126/science.1736354] (in Articles)
...articles Roche eases PCR restrictions M Hoffman Roche eases PCR restrictions. News Animals DNA Fingerprinting methods...on IL-2 were largely right. a RICHARD STONE Roche Eases PCR Restrictions When the powerful gene amplification tech-nique......
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Hoffmann—La Roche's PCR Push
ANN GIBBONSScience 9 August 1991 253: 627 [DOI: 10.1126/science.253.5020.627] (in Articles)
......for the Advancement of Science ARTICLE ARTICLE Hoffmann-La Roche's PCR Push ANN GIBBONS Hoffmann--La Roche's PCR Push. JOURNAL ARTICLE Hoffmann-La Roche's PCR Push While the polymerase chain reaction (PCR) has long been the darling......
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Identification of a peptide specific for Aplysia sensory neurons by PCR-based differential screening
JF Brunet, E Shapiro, SA Foster, ER Kandel, and Y IinoScience 10 May 1991 252: 856-859 [DOI: 10.1126/science.1840700] (in Articles)
......specific for Aplysia sensory neurons by PCR-based differential screening JF Brunet...specific for Aplysia sensory neurons by PCR-based differential screening. In order...Peptide Specific forAplysia Sensory Neurons by PCR-Based Differential Screening JEAN-FRAN0ois......
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And the winner: Cetus does own PCR
M BarinagaScience 8 March 1991 251: 1174 [DOI: 10.1126/science.2006407] (in Articles)
...articles And the winner: Cetus does own PCR M Barinaga And the winner: Cetus does own PCR. News Biotechnology Drug Industry Patents...ANNE SIMON MOFFAT And the Winner: Cetus Does Own PCR Round 1 in a David and Goliath struggle between......
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Biotech nightmare: does Cetus own PCR?
M BarinagaScience 15 February 1991 251: 739-740 [DOI: 10.1126/science.1990435] (in Articles)
...articles Biotech nightmare: does Cetus own PCR? M Barinaga Biotech nightmare: does Cetus own PCR? News Biotechnology economics legislation...United States Biotech Nightmare: Does Cetus Own PCR? The biotech company is locked in combat with......
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PCR analysis of DNA from multiple sclerosis patients for the presence of HTLV-I
CHARLES R.M BANGHAM, SIMON NIGHTINGALE, J.K CRUICKSHANK, and SUSAN DAENKEScience 10 November 1989 246: 821 [DOI: 10.1126/science.2683085] (in Articles)
...articles PCR analysis of DNA from multiple sclerosis patients...NIGHTINGALE J.K CRUICKSHANK SUSAN DAENKE PCR analysis of DNA from multiple sclerosis patients...microbiology Polymerase Chain Reaction ___"Ill W PCR Analysis of DNA from Multiple Sclerosis Patients......
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In Reply: PCR Analysis of DNA from Multiple Sclerosis Patients for the Presence of HTLV-I
E. Premkuwar ReddyScience 10 November 1989 246: 823-824 [DOI: 10.1126/science.246.4931.823] (in Articles)
......Science Article Article In Reply: PCR Analysis of DNA from Multiple Sclerosis...Philadelphia, PA 19104-4268 In Reply: PCR Analysis of DNA from Multiple Sclerosis...Boston, MA 02115 peripheral blood MNCs. PCR reactions were done in a 50-Il volume with......
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In Reply: PCR Analysis of DNA from Multiple Sclerosis Patients for the Presence of HTLV-I
Jennifer H. Richardson, Kai W. Wucherpfenning, N. Endo, Peter Rudge, Angus G. Dalgleish, and David A. HaflerScience 10 November 1989 246: 821-823 [DOI: 10.1126/science.246.4931.821-a] (in Articles)
......Science Article Article In Reply: PCR Analysis of DNA from Multiple Sclerosis...Medical School, Boston, MA 02115 ___"Ill W PCR Analysis of DNA from Multiple Sclerosis...amplifica-tion by the polymerase chain reaction (PCR) of HTLV-I (4) or a related retrovirus......
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Genomic sequencing and methylation analysis by ligation mediated PCR
GP Pfeifer, SD Steigerwald, PR Mueller, B Wold, and AD RiggsScience 10 November 1989 246: 810-813 [DOI: 10.1126/science.2814502] (in Articles)
......methylation analysis by ligation mediated PCR GP Pfeifer SD Steigerwald PR Mueller...ligation mediated polymerase chain reaction (PCR) is used generates high quality, reproducible...methylation analysis by ligation mediated PCR. Genomic sequencing permits studies of......
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In vivo footprinting of a muscle specific enhancer by ligation mediated PCR
PR Mueller and B WoldScience 10 November 1989 246: 780-786 [DOI: 10.1126/science.2814500] (in Articles)
......muscle specific enhancer by ligation mediated PCR PR Mueller B Wold Division of Biology...newly developed polymerase chain reaction (PCR) footprinting procedure. This ligation mediated, single-sided PCR technique permits the exponential amplification......
Polymerase Chain Reaction: Promega Wins Round in Fight Over Taq
Marcia BarinagaScience 23 August 1996 273: 1039-0 [DOI: 10.1126/science.273.5278.1039a] (in News & Comment)
......legislation & jurisprudence Patents Polymerase Chain Reaction Taq Polymerase POLYMERASE CHAIN REACTION Promega Wins Round in Fight Over Taq...the popular technique known as the polymerase chain reaction (PCR)-took a new turn in a San Francisco......
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Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction
P Liang and AB PardeeScience 14 August 1992 257: 967-971 [DOI: 10.1126/science.1354393] (in Articles)
......messenger RNA by means of the polymerase chain reaction P Liang AB Pardee...mRNAs) by means of the polymerase chain reaction. The key element is to...messenger RNA by means of the polymerase chain reaction. Effective methods are......
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Recent advances in the polymerase chain reaction
HA Erlich, D Gelfand, and JJ SninskyScience 21 June 1991 252: 1643-1651 [DOI: 10.1126/science.2047872] (in Articles)
......Recent advances in the polymerase chain reaction HA Erlich D Gelfand...Emeryville, CA 94608. The polymerase chain reaction (PCR) has dramatically...Recent advances in the polymerase chain reaction. The polymerase chain......
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Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain
EY Loh, JF Elliott, S Cwirla, LL Lanier, and MM DavisScience 13 January 1989 243: 217-220 [DOI: 10.1126/science.2463672] (in Articles)
...articles Polymerase chain reaction with single-sided specificity...CA 94305-5402. In the polymerase chain reaction (PCR), two specific oligonucleotide...novel technique, anchored polymerase chain reaction (A-PCR), was devised that......
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