Regular PCR Procedure

General PCR Protocols and Its Product Processes

Recommended Reagent Concentrations

Recommended Reaction Conditions

Initial Conditions

Temperature Cycling

"Hot Start" PCR

Asymmetric PCR for ssDNA Production

Detecting Products

Labelling PCR Products with Digoxigenin

Cleaning PCR Products

Sequencing PCR Products

Cloning PCR Products

AND ALWAYS REMEMBER:

Protocol for PCR using Taq DNA Polymerase
Protocol for PCR with Taq DNA Polymerase. Avoiding Contamination. PCR allows the production of more than 10 million copies of a target DNA sequence from ...www.fermentas.com/techinfo/pcr/dnaamplprotocol.htm -

General PCR Protocol
Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/general_pcr_protocol.html

Standard PCR
However, efficient sequencing of dsDNA generated by normal PCR is possible using the modification to the SequenaseTM protocol published by Bachmann et al. ...www.mcb.uct.ac.za/pcrcond.htm

PCR PROTOCOL
PCR PROTOCOL FOR cDNA ARRAYS ON MEMBRANES. Purpose: to amplify insert DNA from purified plasmid DNA derived from bacterial. plasmid libraries. ...www.daf.jhmi.edu/microarray/protocols/protocol6.pdf

Basic PCR Protocol
Basic PCR Protocol. CGLab, 7/2002. 1). Wipe down the bench area with bleach and a new paper towel. 2). Take the PCR components out of the freezer to thaw ...www.sfsu.edu/~biology/cgl/media/PCR%20Protocol-Basic.pdf

Long PCR Protocol
Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School,Boston, MA, USA.arep.med.harvard.edu/labgc/estep/longPCR_protocol.html

20-mer Polymerase Chain Reaction Procedure (for MJ Research ...
MJ Research thermal Cycler: 10-mer PCR for amplification of random genomic DNA fragments ... Edit (or choose a program if it has been set up) PCR Program. ...wheat.pw.usda.gov/~lazo/methods/lazo/pcrproto.html

Single tube confirmation PCR protocol
For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA.www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

Protocol for PCR with Hot Start Taq DNA Polymerase
Protocol for PCR with Hot Start Taq DNA Polymerase. How to Avoid Contamination. During PCR, usually more than 10 million copies of a template DNA can be ...www.fermentas.com/profiles/modifyingenzymes/pdf/protocols/protocolhotstart.pdf

A Basic Polymerase Chain Reaction Protocol
Here, a basic, straight-forward PCR protocol is. presented. Where appropriate, some of the choices for modifying this standard reaction ...www.idtdna.com/support/technical/TechnicalBulletinPDF/A_Basic_PCR_Protocol.pdf

PCR Reamplification Protocol
PCR Reamplification for Inadequate or Failed Amplifications. Change your standard PCR protocol for the locus as follows:. decrease the number of cycles by ...genome-lab.ucdavis.edu/Protocols/pcr_tips/pcr_reamplification.htm

Inverse PCR and Sequencing Protocol
Inverse PCR and Sequencing Protocol on 5 Fly Preps. For recovery of sequences flanking XP elements. This protocol is an adaptation of ...flystocks.bio.indiana.edu/pdfs/Exel_links/5__fly_iPCR_XP_pub.pdf