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Wednesday, September 10, 2008

PCR RFLP

Restriction fragment length polymorphism (From Wikipedia, the free encyclopedia):
A Restriction Fragment Length Polymorphism (or RFLP, often pronounced as "rif-lip") is a variation in the DNA sequence of a genome which can be detected by a laboratory technique known as gel electrophoresis. Analysis of RFLP variation was an important tool in genome mapping, localization of genetic disease genes, determination of risk for a disease, genetic fingerprinting, and paternity testing.
Contents
1 Analysis technique
2 Examples
3 Applications
4 Alternatives
5 References
5.1 External links

PCR-RFLP Method
18-Oct-02.www.ihwg.org/components/cytokine/mon8Lin.htm

Examples of PCR-RFLP for Nematode Diagnostics
Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes, from University of Nebrasca.nematode.unl.edu/its_id/EXAMPLES/index.htm

Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences
Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences.www.kfunigraz.ac.at/~binder/thesis/node64.html

RFLP/PCR Polymorphism Query Form
Search for RFLP and PCR based polymorphisms by strain, locus symbol, or map position. To search for SNPS, use the SNP Query Form. ...www.informatics.jax.org/searches/polymorphism_form.shtml

PCR, RFLP and Gene Therapy
Lecture 24:Genetic Engineering: PCR, RFLP Analysis & Gene Therapy. The Polymerase Chain Reaction (PCR) Can Make Millions of Copies of DNA in a Short Time ...http://members.aol.com/BearFlag45/Biology1A/LectureNotes/lec24.html

Handbook for DNA isolation, RAPD-PCR and PCR-RFLP
General protocol. We use agarose gels for checking the quality of DNA isolates, PCR products, and PCR-RFLP products, and for scoring RAPD products. ...www.toyen.uio.no/botanisk/brochmann/handbook.htm

PCR-RFLP PROTOCOL FOR ALLELES A AND B PCR ...BOVINE KAPPA-CASEIN PCR-RFLP PROTOCOL. FOR ALLELES A AND B. Laboratory of J.F. Medrano. Department of Animal Science. University of California. ...animalscience.ucdavis.edu/laboratory/

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