Variants of PCR (2)

PCR Serial and It's Related Protocols
PCR (General Procedure)
PCR Primer Design Tools
RT-PCR
Real time PCR
More PCR Protocols Online
Video and Animation of PCR
Mouse Genotyping by PCR
PCR Based Molecular Cloning
PCR Primer Design and Reaction Optimisation
10 Things That Can Kill Your PCR
PCR Troubleshootings


What's Polymerase Chain Reaction (PCR)? ...Principal,Procedure,and more...

The polymerase chain reaction (PCR) is a biochemistry and molecular biology technique[1] forexponentially amplifying a fragment of DNA, via enzymatic replication, without using a living organism (such as E. coli or yeast). PCR can be used for amplification of a single or few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of the DNA piece. As PCR is an in vitro technique, it can be performed without restrictions on the form of DNA, and it can be extensively modified to perform a wide array of genetic manipulations.
Developed in 1983 by Kary Mullis, PCR is now a common technique used in medical and biological research labs for a variety of tasks, such as the sequencing of genes and the diagnosis ofhereditary diseases, the identification of genetic fingerprints (used in forensics and paternity testing), the detection and diagnosis of infectious diseases, and the creation of transgenic organisms. Mullis won the Nobel Prize for his work on PCR.

See: Wikipedia..

FRQs for PCR and other experiments on molecular biology

17. What is PCR? 18. What are some good references for PCR?19. How should I select a set of primers to use for PCR?20. What kinds of programs are available for designing PCR primers?21. What is "Hot-start" PCR? 22. What is AP-PCR or RAPD PCR?23. What is "Touchdown" PCR? 24. Is there a simple method to sequence lambda, M13, or plasmid clones using PCR?
34. Should we break up the methods-reagnts group into subsets with one beingexclusively on the polymerase chain reaction (PCR)?

In situ PCR: protocols and applications

Polymerase Chain Reaction (PCR) Animation

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