Web Source of PCR (2)

Inverse PCR For use with Snyder mTn-lacZ/LEU2 Based Mutagenesis Protocol by M McMurray, Fred Hutchinson Cancer Research Center, Seattle, Wa. USA.http://www.fhcrc.org/labs/gottschling...
Inverse PCR and Cycle Sequencing of P Element Insertions for STS Generation Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.http://www.fruitfly.org/about/methods...
Inverse PCR for PAC-end sequencing To generate PCR fragments that contain the ends of PAC inserts that can be sequenced. Protocol by B Barbazuk, Washington University Zebrafish Genome Resources Project, USA.http://www.genetics.wustl.edu/fish_la...
Inverse PCR protocol step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.http://www.biology.lsa.umich.edu/rese...
Kary B. Mullis - Autobiography The originator of PCR, from the Nobel e-museum web site.http://nobelprize.org/chemistry/laure...
Kary Mullis Inventor Profile of Kary Mullis, the originator of PCR, from the National Inventors Hall of Fame web site.http://www.invent.org/hall_of_fame/10...
Life at High Temperatures Article by TD Brock from the web site of Department of Bacteriology, University of Wisconsin - Madison on the thermophilic organisms in Yellowstone hot springs, including Thermus aquaticus from which Taq polymerase was obtained.http://www.bact.wisc.edu/Bact303/b27
Long PCR Protocol Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USAhttp://twod.med.harvard.edu/labgc/est...
MJ Research Manufactures and sells products for molecular biology including thermal cyclers and sequencers / genotypers.http://www.mjr.com/
Molecular Staging, Inc. Company promoting diagnostic tests for genetic analysis; rolling circle amplification technology (RCAT), developed for amplifying signals from DNA, RNA and proteins, from Westport, CT.http://www.molecularstaging.com/Pages...
Mutation Detection Systems - SSCP Analysis Discussion of the technique and variations, literature references. By S McQuaid, Institute of Child Health, University College London, UK.http://www.ich.ucl.ac.uk/cmgs/sscp.htm
Nematode ITS1 Size Variation Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes.http://nematode.unl.edu/its_id/EXAMPL...
Optimizing Multiplex and LA-PCR with Betaine LA-PCR = "long and accurate PCR". Article by PN Hengen in TIBS June 1997.http://www-lecb.ncifcrf.gov/~pnh/pape...
Optimizing PCR Protocols Brief guidelines. From the Jackson Laboratory, University of Maine, USA.http://www.jax.org/imr/optimize_pcr.html
PCR Amplification of cDNA Segments by 2 Stage Nested PCR Protocol from the method database of NIH, USA.http://www.ncbi.nlm.nih.gov/SNP/snp_v...
PCR Project Presentations from the University of California at Berkley on PCR, both current research reports and reviews.http://sunsite.berkeley.edu/biotech/pcr/
PCR Protocol Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.http://www.biology.lsa.umich.edu/rese...
PCR Protocols Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State Universityhttp://www.cas.psu.edu/docs/CASDEPT/V...
PCR Technology An introduction by Connie Veilleux from the US National Health Museum website.http://www.accessexcellence.org/LC/SS...
PCR Troubleshooting Limited to conventional straight forward PCR. Page designed and maintained by Octavian Henegariu on the web site of the Yale - New Haven Medical Center.http://info.med.yale.edu/genetics/war...
PCR and multiplex PCR guide Discussions of the parameters influencing the PCR reaction and some PCR and multiplex PCR applications, by Octavian Henegariu on the web site of the Yale - New Haven Medical center.http://www.info.med.yale.edu/genetics...
PCR animated Animation illustrating the principle of PCR, from the University of Ghent, Belgium.http://allserv.rug.ac.be/~avierstr/pr...
PCR method protocols Protocols for PCR posted by the Helen Donis-Keller Laboratory.http://hg.wustl.edu/hdk_lab_manual/pc...
PCR primer design and reaction optimisation Article by Ed Rybicki, Department of Molecular and Cell Biology, University of Cape Town in: Molecular Biology Techniques Manual, on the web site of the University of Cape Town.http://web.uct.ac.za/microbiology/pcr...
PCR protocols Brief protocols for PCR in use at the Genome Sequencing Center of the Washington University in St Louis Medical School.http://genome.wustl.edu/tools/protoco...
Polymerase Chain Reaction Popular survey article by Mark V. Bloom, DNA Learning Center, Cold Spring Harbor Laboratory, from the web site of the US National Health Museum.http://www.accessexcellence.com/RC/CT...