Variants of PCR (3)

A Rapid DNA Minipreparation Method Suitable for AFLP and Other PCR Applications (PDF document) - Preparation of DNA from plant tissues suitable for PCR methods including AFLP, article by DH CHEN and PC RONALD Department of Plant Pathology, University of California, Davis.

Adjuvants in PCR Reactions - Brief discussion of additives to improve amplification efficiency and specificity of PCR, by Octavian Henegariu, Yale-New Haven Medical Center.

Alu-PCR Hybridization - A report and protocol, from Fondation Jean Dausset.

Anchor Probes for Comparative Mapping of Grass Species - Article in which probes from different libraries were used to hybridize seven cereals at the Department of Plant Breeding and Biometry, Cornell University, NY.

Attotron Biosensor Corporation - R & D company for development of biosensors and related products for the research and educational markets.

BioRad, Amplification, PCR - Division of BioRad Laboratories that manufactures and sells instruments for PCR, in Hercules, California, USA.

Biosource molecular methods booklet - 56 page booklet on oligonucleotides, PCR and RT-PCR, from Biosource (company).

C. elegans Gene Knockout Project - Protocols including standard nested PCR, from the Biotechnology Laboratory, University of British Columbia.

Degenerate PCR - An introduction with principle, applications and description of the technique. From the web site of Kebangsaan University, Malaysia.

Degenerate PCR - The identification of novel members of gene families by PCR using degenerate primers is described and protocols given. Article by Michael Koelle 1996 on the web site of Dartmouth College.

Design Oligonunucleotide Primers - Interface for scanning DNA sequences for restriction sites, without changing encoded protein created. Includes FAQ, table of enzymes and links to tools, maintained at the University of Waterloo, ON, Canada.

Detection of Asymmetric PCR Products in Homogeneous Solution by Fluorescence Correlation Spectroscopy - In asymmetric PCR, the low concentration primer is quantitatively incorporated into double stranded DNA after an appropriate number of cycles. If this primer is fluorescent labelled, the dsDNA can be quantitated from the fluorescence.

Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences - RFLP = Restriction Fragment Length Polymorphism. By Alexander Binder 1997.

Detection of Single Nucleotide Mutations in Wheat Using Single Strand Conformation Polymorphism Gels (PDF file) - P Martins-Lopez, H Zhang, R Koebner, Plant Mol. Biol. Reporter 19(2001): 159-162. From National Research Coouncil Canada.

DNALC: PCR Animation - An animation explaining how the Polymerase Chain Reaction (PCR) works, from the Dolan DNA learning center, Cold Spring Harbor Laboratory, USA.

Dolan DNA Learning Centers Gene Almanac - Educational site on topics in genetics and gene expression from Cold Spring Harbor Laboratory, USA.

Effect of PCR Buffer on multiplex PCR (PDF) - Multiplex PCR employs different primer pairs in the same amplification reaction. This requires extensive optimization of annealing conditions. From Quiagen (company)

Fidelity of DNA Polymerases for PCR - Article by PN Hengen from TIBS 1995

FISH Guide and Troubleshooting - Links to pages describing influential parameters, with guides on PCR, RT-PCR and multiplex PCR reactions, Taq, FISH, CM-FISH, TM-FISH, microarrays, CCK, slide prep and labeling, maintained by Octavian Henegariu from Yale University, New Haven, CT.

GeneOhm Sciences - Tests on gruup B Streptococcus and methicillin resistant Staphylococcus aureus by PCR / DNA sequencing.

GenHunter - Manufacturer of material for differential display PCR in Nashville, Tenn USA.

HiFi DNA - HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.

Ingenetix GmbH - Develops technology and products for DNA and mRNA research. Also provide DNA testing for the determination of parentage/paternity and custom DNA sequencing, oligonucleotide synthesis, genotyping services, pharmacogenetics and quantitative PCR, in Vienna, Austria.

Inverse PCR and Cycle Sequencing of P Element Insertions for STS Generation - Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.

Inverse PCR for PAC-end sequencing - To generate PCR fragments that contain the ends of PAC inserts that can be sequenced. Protocol by B Barbazuk, Washington University Zebrafish Genome Resources Project, USA.

Inverse PCR For use with Snyder mTn-lacZ/LEU2 Based Mutagenesis - Protocol by M McMurray, Fred Hutchinson Cancer Research Center, Seattle, Wa. USA.

Inverse PCR protocol - step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.

Kary B. Mullis - Autobiography - The originator of PCR, from the Nobel e-museum web site.

Kary Mullis - Inventor Profile of Kary Mullis, the originator of PCR, from the National Inventors Hall of Fame web site.

Long PCR Protocol - Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA

Nematode ITS1 Size Variation - Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes, from University of Nebrasca.

Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler - Basic discussion of the optimization of PCR, from the web site of Brinkmann Instruments.

Optimizing Multiplex and LA-PCR with Betaine - LA-PCR = "long and accurate PCR". Article by PN Hengen in TIBS June 1997.

Optimizing PCR Protocols - Brief guidelines. From the Jackson Laboratory, University of Maine, USA.


PCR Amplification of cDNA Segments by 2 Stage Nested PCR - Protocol from the method database of NIH, USA.

PCR and multiplex PCR guide - Discussions of the parameters influencing the PCR reaction and some PCR and multiplex PCR applications, by Octavian Henegariu on the web site of the Yale - New Haven Medical center.