Web Source of PCR (3)

Polymerase Chain Reaction (PCR) A graphic description of the principle of PCR from the US National Health Museum web site.http://www.accessexcellence.org/AB/GG/polymerase.html
Principle of PCR Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium.http://allserv.rug.ac.be/~avierstr/principles/pcr.html
Protocol for T-RFLP by Capillary Electrophoresis Detailed protocol for terminal restriction fragment length polymorphism (T-RFLP), which allows the fingerprinting of a community by analyzing the polymorphism of a certain gene. Article by V Grntzig, B Stres, HL Ayala del Ro and JM Tiedje, Center for Microbial Ecology, Michigan State University, USAhttp://rdp.cme.msu.edu/html/t-rflp_jul02.html
Protocols Online: PCR Protocols Extensive collection of PCR protocols and methods from Protocol On Line.http://www.protocol-online.org/prot/Molecular_Biology/PCR/
Quantitative PCR Protocol From the Jackson Laboratory, University of Maine, USA.http://www.jax.org/cyto/quanpcr.html
RACE amplification Rapid Amplification of cDNA Ends. Protocol, from National Institute of Genetics, Japan.http://www.nig.ac.jp/labs/EarlyEmb/midline/protocol/RACE.html
RAPD PCR RAPD stands for Random Amplification of Polymorphic DNA, where the target sequence(s) (to be amplified) is unknown.Brief description, from Rutgers University, USA.http://avery.rutgers.edu/WSSP/StudentScholars/project/archives/onions/rapd.html
RAPD analysis with P. infestans Protocol for Randomly Amplified Polymorphic DNA, by H Judelson, University of California, Riverside, USA.http://www.fgsc.net/methods/Judel.html
RAPD's Brief description of Random Amplified Polymorphic DNA, from Oklahoma State University, USA.http://opbs.okstate.edu/~melcher/MG/MGW1/MG11127.html
RFLP Definition RFLP = Restriction Fragment Length Polymorphism, from FDAhttp://vm.cfsan.fda.gov/~frf/rflp.html
Random Arbitrarily Primed PCR Protocol for RAP-PCR to study prokaryotic gene expression, from the Enterococcus Research Site, University of Oklahoma, USA.http://www.enterococcus.ouhsc.edu/random_pcr.asp
Rational primer design greatly improves differential display-PCR (DD-PCR) Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.http://nar.oupjournals.org/cgi/content/full/25/11/2239
Reference in PCR Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers.http://www.gene-quantification.org/
Rep-PCR Genomic Fingerprinting Bacteria are characterized by Rep-PCR fingerprinting using primers corresponding to naturally occurring repetitive sequences in the interspersed regions.http://www.msu.edu/user/debruijn/
Roe Laboratory Protocols Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.http://www.genome.ou.edu/proto.html
Single tube confirmation PCR protocol For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA.http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html
Standard PCR protocols From Molecular Biology Techniques Manual, from the web site of the University of Cape Town, South Africa.http://web.uct.ac.za/microbiology/pcrcond.htm
T-DNA Generated Enhancer Traps in Arabidopsis Application of inverse PCR, partial genomic libraries and TAIL-PCR in cloning flanking, at the Department of Biological Sciences, Dartmouth College, Hanover, NH.http://www.dartmouth.edu/~tjack/
Tavi's PCR protocols A page describing the main parameters and trouble-shooting in PCR. The page is somewhat dated (updated 1997) but still useful.http://info.med.yale.edu/genetics/ward/tavi/PCR.html
Technical manual of PCR Intended for specialists planning PCR procedures in their laboratories. From the University of Cape Town updated 2001.http://web.uct.ac.za/microbiology/pcr.htm
The Polymerase Chain Reaction A popular description of the way it works, its importance in different connections, legal aspects. Authored by TM Powledge at the Federation of the American Societies of Experimental Biology.http://www.faseb.org/opar/bloodsupply/pcr.html
The web guide of PCR List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina.http://www.pcrlinks.com/
Thermostable DNA Polymerases Discussion of their origin and briefly their properties. From the web site of Colorado State University.http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/enzymes/hotpolys.html
Wayward PCR primers Article by PN Hengen from TIBS 1995 on the loss of activity of PCR primers with time.http://www-lecb.ncifcrf.gov/~pnh/papers/TIBS/jan95.html
What the Heck is PCR? Popular description of the PCR technique by John C Brown, University of Kansas 1995.http://people.ku.edu/~jbrown/pcr.html
Which DNA Marker for Which Purpose? Compendia of the Research Project "Development, optimisation and validation of molecular tools for assessment of biodiversity in forest trees", European Union DGXII Biotechnology FW IV Research Programme. From the web site of the University Library, Gttingen.http://webdoc.sub.gwdg.de/ebook/y/1999/whichmarker
quot; Technique for Enhancing Detection of Small Deletions in Mutant Libraries This method works because the poison allows the formation of deletion products but titers out full-sized products. From the Biotechnology Laboratory, University of British Columbia.http://ko.cigenomics.bc.ca/poison1.html