RAPD PCR

RAPD PCR
RAPD stands for Random Amplification of Polymorphic DNA, where the target sequence(s) (to be amplified) is unknown.Brief description, from Rutgers ...avery.rutgers.edu/WSSP/StudentScholars/project/archives/onions/rapd.html

RAPD PCR Reaction Mixes:
Type, Initialize, X, Denature, Anneal, Extend, Termination. RAPD 10-mer, 95C, 5min, 45, 94C, 0:30, 35C, 1:00, 72C, 2:00, 72C, 5:00. PCR 18+mer, 95C, 5:00 ...wheat.pw.usda.gov/~lazo/methods/lazo/pcrmeth0.html

Handbook for DNA isolation, RAPD-PCR and PCR-RFLP
A standard RAPD-PCR reaction is performed in volumes of 25.0 m l containing 50 mM KCl, 10 mM Tris-HCl pH 9.0, 1.5 mM MgCl2, 0.001% gelatine, ...www.toyen.uio.no/botanisk/brochmann/handbook.htm

RAPD Protocol dNTP mix (5mM): Loading buffer (6X): Ladder mix: PCR .RAPD Protocol. Protocol for Promega. and Stoffel brand Taq. enzymes (1 reaction):. 4 µl dNTP (5mM) mix. 2.5 µl 10X PCR buffer. 8.33 µl H ...www.css.msu.edu/bean/PDF/RAPD_protocol.pdf

PCR PROTOCOL FOR RAPDs--1PCR PROTOCOL FOR RAPDs. Application: Fingerprinting, molecular mapping, ... Amount of DNA needed per RAPD reaction according to the plant species under ...gene4.ciat.cgiar.org/limsys3.0/Modelos/protocolos/PCRPROTOCOLFORRAPDs.doc