Long PCR Protocols

Kyle’s protocol for long PCR
Kyle’s protocol for long PCR. This protocol intended for generation of long PCR products (3-10kb) from mouse ES cell or tail DNA. General Considerations: ...www.whitelabs.org/Lab%20Protocols/PCR%20Protocols/kyle.htm
Long PCR Protocol
Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA.arep.med.harvard.edu/labgc/estep/longPCR_protocol.html
Tips for Long and Accurate PCR Tips for Long and Accurate PCR. Wayne M. Barnes. Department of Biochemistry and Molecular Biophysics, Washington University School of ...http://www.klentaq.com/products/lapcrchapter.pdf
Mullins Lab : Protocols : Long PCR
Two long PCR steps:. First round of the nested PCR step of the end point dilution procedure to quantitate the cDNA. First round of the nested PCR step of ...ubik.microbiol.washington.edu/protocols/other/longpcr.htm
Long PCR
LONG PCR AMPLIFICATION OF THE FVIII GENE INTRON 22 GENE INVERSION. Introduction. Long range PCR allows the amplification of PCR products, which are much ...europium.csc.mrc.ac.uk/WebPages/Database/Methods/longpcr.htm
Extra-Long PCR Ki, EPICENTRE Biotechnologies
EPICENTRE develops and manufactures reagents and kits for genomics, proteomics, and RNA research. EPICENTRE's products are used worldwide in a broad range ...www.epibio.com/item.asp?ID=306
Long PCR Protocol
Efficient Long-PCR results from the use of two polymerases: a non-proofreading polymerase is the main polymerase in the reaction, and a proofreading ...wheat.pw.usda.gov/~lazo/methods/lazo/longpcr.html
Long PCR.
Long PCR. Cheng S, Chang SY, Gravitt P, Respess R. Department of Human Genetics, Roche Molecular Systems, Inc., Alameda, California 94501. Mesh Terms: ...www.ncbi.nlm.nih.gov/pubmed/8208299
Long PCR Product Sequencing (LoPPS): a shotgun-based approach to sequence long PCR products.
Here we describe a practical procedure for sequencing long PCR products. The method relies on ultrasonic shearing of PCR products, resulting in fragments ...www.nature.com/nprot/journal/v2/n2/abs/nprot.2006.453.html
Long PCR Protocol
Aug 10, 2003 ... Introduction · Research Pages · People involved Photo galleries Publications Other sample sites Education · Links to other micobial ...www.hawaii.edu/microbiology/MO/longpcr.htm
Long PCR -- Sambrook and Russell.
Primers used for long PCR are generally slightly longer (25-30 nucleotides) than those used for standard PCR. It is particularly important to strive for ...www.cshprotocols.org/cgi/content/full/2006/2/pdb.prot3841
Efficient Cloning of Long PCR Inserts with the In-Fusion PCR Cloning System.Clontech’s In-Fusion PCR cloning method. provides superior results when applied to the. challenging task of cloning long PCR fragments. ...www.clontech.com/images/ctq/APR07UPD/CR742319_LongPCR_IF_US.pdf
Nature Protocols: Long PCR amplification of the entire mitochondrial genome from individual helminths for direct sequencing.
In this article, we describe a practical long PCR approach for the amplification and subsequent sequencing of the entire mt genome from individual helminths ...www.natureprotocols.com/2007/09/20/long_pcr_amplification_of_the.php
Long PCR - MyBio
Long PCR Reagents and GuidelinesLong PCR Reagents and Guidelines. ... General Guidelines for Long PCR Conditions and Enzyme Mixtures Efficient Long PCR ...mybio.wikia.com/wiki/Long_PCR